کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4201940 1279433 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning and Expression of Recombinant Tick-Borne Encephalitis Virus-like Particles in Pichia pastoris
ترجمه فارسی عنوان
کلونینگ و بیان عصاره های مشابه ویروس مشابه با تکرار مجدد آن در پنچیا پاستریس
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی سیاست های بهداشت و سلامت عمومی
چکیده انگلیسی

ObjectivesThe purpose of this study was to verify the feasibility of using the glyceraldehyde-3-phosphate dehydrogenase (GAP) promotor based Pichia pastoris expression system to produce tick-borne encephalitis virus (TBEV) virus-like particles (VLPs).MethodsThe complementary DNA encoding the TBEV prM signal peptide, prM, and E proteins of TBEV Korean strain (KrM 93) was cloned into the plasmid vector pGAPZɑA, then integrated into the genome of P. pastoris, under the control of the GAP promoter. Expression of TBEV VLPs was determined by Western blotting using monoclonal antibody against TBEV envelope (E) protein.ResultsRecombinant TBEV VLPs consisting of prM and E protein were successfully expressed using the GAP promoter-based P. pastoris expression system. The results of Western blotting showed that the recombinant proteins were secreted into the culture supernatant from the P. pastoris and glycosylated.ConclusionThis study suggests that recombinant TBEV VLPs from P. pastoris offer a promising approach to the production of VLPs for use as vaccines and diagnostic antigens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Osong Public Health and Research Perspectives - Volume 5, Issue 5, October 2014, Pages 274–278
نویسندگان
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