کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4269557 1610851 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of a Tadalafil Analogue as an Adulterant in a Dietary Supplement for Erectile Dysfunction
ترجمه فارسی عنوان
تشخیص یک آنالوگ تادالافیل به عنوان یک مکمل غذایی در مکمل غذایی برای اختلال نعوظ
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی اورولوژی
چکیده انگلیسی

IntroductionSeveral cases of adulteration of dietary supplements with tadalafil, sildenafil, and vardenafil, or their unapproved analogues have been reported worldwide. Mainly, the presence of the latter represents a serious health risk to consumers as their efficacy and toxic effects have not been assessed and may result in unpredictable adverse effects.AimTo investigate the suspected adulteration with synthetic phosphodiesterase type 5 (PDE‐5) inhibitors in a dietary supplement marketed in Argentina for the treatment of erectile dysfunction (ED).MethodsThe content of the capsules of the dietary supplement (sample A) was analyzed by thin layer chromatography (TLC) and high‐performance liquid chromatography (HPLC) diode‐array detection. From the organic extract of sample A, a major compound was purified by column chromatography (CC). The isolated compound was identified by proton nuclear magnetic resonance (1H NMR) and carbon NMR (13C NMR), heteronuclear single quantum coherence, distortionless enhancement by polarization transfer (DEPT 135), electrospray ionization mass spectrometry, and ultraviolet, and infrared (Fourier transform infrared spectroscopy) spectroscopy.Main Outcome MeasureProof of adulteration of herbal products with synthetic PDE‐5 inhibitors.ResultsBy TLC and HPLC analysis, a major compound was detected in sample A organic extract. The purification of this extract by CC led to the isolation of a pure compound which was identified according to its spectral data as (6R,12aR)‐2‐amino‐6‐(1,3‐benzodioxol‐5‐yl)‐2,3,6,7,12,12a‐hexahydropyrazino [1′,2′:1,6] pyrido [3,4‐b] indole‐1,4‐dione or aminotadalafil.ConclusionsAn unapproved PDE‐5 inhibitor analogue, which was identified as aminotadalafil, has been detected in a dietary supplement. This study represents the first report in Latin America and one of the few independent studies of an adulteration with an unapproved PDE‐5 inhibitor of an herbal product for ED treatment. Ulloa J, Sambrotta L, Redko F, Mazza ON, Garrido G, Becher EF, and Muschietti L. Detection of a tadalafil analogue as an adulterant in a dietary supplement for erectile dysfunction. J Sex Med 2015;12:152–157.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Sexual Medicine - Volume 12, Issue 1, January 2015, Pages 152–157
نویسندگان
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