کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4300998 1612528 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Energy status of pig donor organs after ischemia is independent of donor type
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Energy status of pig donor organs after ischemia is independent of donor type
چکیده انگلیسی

BackgroundLiterature is controversial whether organs from living donors have a better graft function than brain dead (BD) and non–heart-beating donor organs. Success of transplantation has been correlated with high-energy phosphate (HEP) contents of the graft.MethodsHEP contents in heart, liver, kidney, and pancreas from living, BD, and donation after cardiac death in a pig model (n = 6 per donor type) were evaluated systematically. BD was induced under general anesthesia by inflating a balloon in the epidural space. Ten hours after confirmation, organs were retrieved. Cardiac arrest was induced by 9 V direct current. After 10 min of ventricular fibrillation without cardiac output, mechanical and medical reanimation was performed for 30 min before organ retrieval. In living donors, organs were explanted immediately. Freeze-clamped biopsies were taken before perfusion with Celsior solution (heart) or University of Wisconsin solution (abdominal organs) in BD and living donors or with Histidine-Tryptophan-Ketoglutaric solution (all organs) in non–heart-beating donors, after perfusion, and after cold ischemia (4 h for heart, 6 h for liver and pancreas, and 12 h for kidney). HEPs (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, and phosphocreatine), xanthine, and hypoxanthine were measured by high-performance liquid chromatography. Energy charge and adenosine triphosphate-to-adenosine diphosphate ratio were calculated.ResultsAfter ischemia, organs from different donor types showed no difference in energy status. In all organs, a decrease of HEP and an increase in hypoxanthine contents were observed during perfusion and ischemia, irrespective of the donor type.ConclusionOrgans from BD or non–heart-beating donors do not differ from living donor organs in their energy status after average tolerable ischemia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 180, Issue 2, April 2013, Pages 356–367
نویسندگان
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