کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4301489 1288439 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Changes in morphology and function in small intestinal mucosa after Roux-en-Y surgery in a rat model
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Changes in morphology and function in small intestinal mucosa after Roux-en-Y surgery in a rat model
چکیده انگلیسی

BackgroundCurrently there is no an appropriate model to study intestinal mucosal atrophy in vivo that preserves the nutritional status of the organism.Materials and methodsWe created a defunctionalized segment of jejunum via a dead-end Roux-en-Y anastomosis in rats. We compared tissue morphometric parameters in the intestinal mucosa of the defunctionalized bowel with that of the mucosa proximal and distal to the anastomosis. We further measured extracellular signal-regulated kinase (ERK) activation within the mucosa as well as sucrase-isomaltase and dipeptidyl peptidase-4 levels as markers of intestinal mucosal differentiation by Western blotting of mucosal scrapings.ResultsThree days after anastomosis, the defunctionalized bowel exhibited decreased diameter and thickness of both the mucosa and the fibromuscular layer compared with adjacent bowel in continuity for luminal nutrient flow or with bowel from control animals. Sucrase-isomaltase and dipeptidyl peptidase-4 levels also were decreased. Furthermore, mucosal ERK activation, assessed as the ratio of phosphorylated to total ERK, also was reduced. Animal weights did not differ between bypassed and control animals.ConclusionsDeprivation of nutrient flow in a segment of bowel by defunctionalizing Roux-en-anastomosis produces mucosal atrophy as indicated by altered histology, differentiation marker expression, and ERK signaling, in animals that are otherwise able to maintain enteral nutrition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 177, Issue 1, September 2012, Pages 63–69
نویسندگان
, ,