Pterostilbene Inhibits Lung Cancer Through Induction of Apoptosis1

BackgroundLung cancer remains the leading cause of cancer mortality in the United States. Resveratrol is a potent antioxidant found in grapes that inhibits several types of cancer, including lung cancer. Herein, we investigated the effects of pterostilbene, an analog of resveratrol found in blueberries, on lung cancer, in vitro. We hypothesized that pterostilbene would inhibit lung cancer cell growth in vitro by a pro-apoptotic mechanism.MethodsTwo lung cancer cell lines (NCI-H460 and SK-MES-1) were cultured using standard techniques. Cells were treated with increasing doses of pterostilbene (10–100μM). Cell viability was measured at 24, 48, and 72 h using a MTT assay. Apo-ONE Caspase-3/7 assay was used to evaluate caspase activity. T-test and two-way ANOVA were used for statistical analysis.ResultsPterostilbene significantly decreased cell viability in lung cancer cells in a concentration- and time-dependent manner (P < 0.001). Concentrations greater than 20 μM of pterostilbene produced significant growth inhibition by 72 h (P < 0.001). Apoptosis and caspase-3/7 activity were significantly increased by pterostilbene treatment (P < 0.05).ConclusionsPterostilbene inhibits growth via apoptosis induction in vitro. Further in vitro mechanistic studies and in vivo experiments are warranted to determine the potential role for pterostilbene in lung cancer treatment or prevention.