کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4303108 1612530 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ethyl Pyruvate Prevents Intestinal Inflammatory Response and Oxidative Stress in a Rat Model of Extrahepatic Cholestasis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Ethyl Pyruvate Prevents Intestinal Inflammatory Response and Oxidative Stress in a Rat Model of Extrahepatic Cholestasis
چکیده انگلیسی

BackgroundRinger's ethyl pyruvate solution (REPS) has been shown to ameliorate liver injury in a murine model of extrahepatic cholestasis. The goal of the present investigation was to gain additional information about whether infusing REPS instead of Ringer's lactate solution (RLS) after inducing obstructive jaundice would be beneficial to intestinal barrier function, inflammatory response, and oxidative stress.MethodsMale Sprague Dawley rats were divided into three groups: Group Sham (n = 6), sham-treated controls; Group RLS (n = 9), common bile duct ligation (CBDL) plus RLS; and Group REPS (n = 9), CBDL plus REPS. On 14 d after BDL, the rats were sacrificed and intestinal permeability was analyzed. Ileal IL-6 and TNF-α levels, malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO), and NF-κB activity were determined. Histologic examination and apoptosis of ileum were also examined.ResultsRelative to sham-treated controls, CBDL in RLS-treated rats were associated with increased intestinal permeability to FITC-labeled dextran (4.51 ± 0.85 versus 0.44 ± 0.18, P < 0.01), histopathologic damage and apoptosis (68.4 ± 13.4 versus 6.7 ± 1.9 pre-1000 villi cells, P < 0.01). IL-6 and TNF-α level, MDA, MPO, and NF-κB activity in ileal tissues were also promoted, along with decreased GSH levels. Treatment with REPS significantly decreased intestinal permeability (3.37 ± 0.71, P < 0.01) and apoptosis (42.8 ± 14.3 pre-1000 villi cells, P < 0.01). Other changes were also significantly attenuated by treatment with REPS after CBDL.ConclusionsThe present study demonstrates that administration of REPS, but not RLS, maintains intestinal barrier function and reduces intestinal oxidative damage, inflammatory response, and apoptosis in cholestatic rats. This effect of ethyl pyruvate may be useful for preventing intestinal injury in patients with biliary obstruction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 160, Issue 2, 15 May 2010, Pages 228–235
نویسندگان
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