کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4303611 1288483 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PKC and MLCK-Dependent, Cytokine-Induced Rat Coronary Endothelial Dysfunction
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
PKC and MLCK-Dependent, Cytokine-Induced Rat Coronary Endothelial Dysfunction
چکیده انگلیسی

BackgroundHeart disease is one of the leading causes of death in the United States, killing nearly one million people every year. Inflammatory mediators or cytokines are released following myocardial infarction and ischemia/reperfusion injury. These cytokines, of which interleukin-1β, interleukin-6, and tumor necrosis factor-alpha (TNF-α) are among the most important, propagate the activation of a multitude of signaling pathways, such as the protein kinase C (PKC) and myosin light chain kinase (MLCK) pathways, which lead to deleterious changes in the structure and function of the coronary microvascular endothelium.MethodsThe effects of cytokines on rat heart microvascular endothelial cell monolayer integrity, PKC activity, and adherens junction protein alteration were examined. Further, an in vivo rat coronary ischemia/reperfusion injury model was used to determine vascular leakage and TNF-α release.ResultsAdministration of the above mentioned cytokines to cell monolayers resulted in significant increases in PKC activation, gap formation, and hyperpermeability across the monolayer and β-catenin phosphorylation/reorganization. Inhibition of conventional PKC and MLCK attenuated permeability increases. Ischemia/reperfusion injury to the left ventricle resulted in TNF-α release as well as conventional PKC- and MLCK-dependent protein extravasation from the circulation to the heart tissue.ConclusionThese results identify the conventional PKC and MLCK pathways as important factors in coronary endothelial dysfunction elicited by IR injury and cytokine release. Further examination of these molecular signaling cascades has the potential of identifying targets for therapeutic intervention following ischemic events in the heart.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 152, Issue 1, March 2009, Pages 76–83
نویسندگان
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