کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4304553 1288507 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Postischemic Infusion of 17-β-Estradiol Protects Myocardial Function and Viability 1
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Postischemic Infusion of 17-β-Estradiol Protects Myocardial Function and Viability 1
چکیده انگلیسی

BackgroundFemales demonstrate improved cardiac recovery after ischemia/reperfusion injury compared with males. Attenuation of myocardial dysfunction with preischemic estradiol suggests that estrogen may be an important mediator of this cardioprotection. However, it remains unclear whether post-injury estradiol may have clinical potential in the treatment of acute myocardial infarction. We hypothesize that postischemic administration of 17β-estradiol will decrease myocardial ischemia/reperfusion injury and improve left ventricular cardiac function.Materials and methodsAdult male Sprague Dawley rat hearts (n = 20) (Harlan, Indianapolis, IN) were isolated, perfused with Krebs-Henseleit solution via Langendorff model, and subjected to 15 min of equilibration, 25 min of warm ischemia, and 40 min reperfusion. Experimental hearts received postischemic 17β-estradiol infusion, 1 nm (n = 4), 10 nm (n = 4), 25 nm (n = 4), or 50 nm (n = 4), throughout reperfusion. Control hearts (n = 4) were infused with perfusate vehicle.ResultsPostischemic recovery of left ventricular developed pressure was significantly greater with 1 nm (51.6% ± 7.4%) and 10 nm estradiol (47.7% ± 8.6%) than with vehicle (37.8% ± 9.7%) at end reperfusion. There was also greater recovery of the end diastolic pressure with 1 nm (47.8 ± 4.0 mmHg) and 10 nm estradiol (54.0 ± 4.0) compared with vehicle (75.3 ± 7.5). Further, 1 nm and 10 nm estrogen preserved coronary flow after ischemia and decreased coronary effluent lactated dehydrogenase compared with controls. Estrogen at 25 nm and 50 nm did not provide additional benefit in terms of functional recovery. Estrogen at all concentrations increased extracellular signal-regulated protein kinase phosphorylation.ConclusionsPostischemic infusion of 17β-estradiol protects myocardial function and viability. The attractive potential for the clinical application of postischemic estrogen therapy warrants further study to elucidate the mechanistic pathways and differences between males and females.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 146, Issue 2, 15 May 2008, Pages 218–224
نویسندگان
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