The role of Homer 1a in increasing locomotor activity and non-selective attention, and impairing learning and memory abilities

• We studied the role of Homer 1a in ADHD (attention deficit hyperactivity disorder).
• RNAi (RNA interference) was used to silence Homer 1a expression.
• Some behaviors related to ADHD were observed in the RNAi rat group.
• These behaviors tended to normalize after treatment with MPH (methylphenidate).
• This supports the link between Homer 1a and the etiology and pathogenesis of ADHD.

The current study aimed to investigate the possible role of Homer 1a in the etiology and pathogenesis of attention deficit hyperactivity disorder (ADHD). We divided 32 rats into four groups. The rats in the RNAi-MPH group were given the lentiviral vector containing Homer 1a-specific miRNA (Homer 1a-RNAi-LV) by intracerebroventricular injection, and 7 days later they were given three daily doses of methylphenidate (MPH) by intragastric gavage. The RNAi-SAL group was given Homer 1a-RNAi-LV and saline later. The NC-MPH group was given the negative control lentiviral vector (NC-LV) and MPH later. The NC-SAL group was given NC-LV and saline later. Rats that were given Homer 1a RNAi exhibited increased locomotor activity and non-selective attention, and impaired learning and memory abilities, which is in line with the behavioral findings of animal models of ADHD. However, MPH ameliorated these abnormal behaviors. All findings indicated that Homer 1a may play an important role in the etiology and pathogenesis of ADHD.