کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4325498 1614008 2012 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Presynaptic residual calcium and synaptic facilitation at hippocampal synapses of mice with altered expression of SNAP-25
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Presynaptic residual calcium and synaptic facilitation at hippocampal synapses of mice with altered expression of SNAP-25
چکیده انگلیسی

Paired pulse facilitation (PPF) is a form of short-term synaptic plasticity that results from an interaction of residual presynaptic Ca2 + ([Ca2 +]res), number of release-competent vesicles, and the sensitivity of the vesicle release mechanisms to Ca2 +. While PPF is predominant at hippocampal Schaffer collateral-CA1 (SC-CA1) synapses, facilitation is greater in adult mice (designated Tkneo) that over express an isoform of the plasma membrane-targeted SNARE protein, SNAP-25a, which is normally predominantly expressed in juvenile animals. SNAP-25 is essential for action potential-dependent neuroexocytosis, yet the significance of the shift between the alternatively spliced variants SNAP-25a and SNAP-25b is not fully understood. This alteration of a key component of the protein machinery required for neurotransmitter release in Tkneo mice, therefore, provides a useful tool to further investigate presynaptic mechanisms that influence short-term plasticity. To explore this link between SNAP-25 and PPF, we simultaneously measured postsynaptic potentials and presynaptic [Ca2 +]res during paired-pulses in adult Tkneo, heterozygote null (HET), and wild type (WT) mice. We demonstrate that enhanced PPF is maintained at mature hippocampal synapses of Tkneo mice that predominantly express SNAP-25a, and that [Ca2 +]res kinetics are altered at synapses of Tkneo and HET mice, both of which exhibit reduced levels of total SNAP-25 expression. To evaluate the role of SNAP-25 in short-term plasticity and [Ca2 +]res regulation, we applied a vesicular release probability model for neurotransmission. Our results suggest that the isoform expression and total level of SNAP-25 affect both [Ca2 +]res dynamics and the ability of releasable vesicles to enter into a facilitated state.


► Facilitation and [Ca2 +]res were studied in SNAP-25a overexpressing mutant mice.
► SNAP-25 isoform or total level did not affect initial fEPSP amplitude.
► SNAP-25 isoform affected [Ca2 +]res during the first and second of paired pulses.
► Modeling studies suggest a role of SNAP-25 in [Ca2 +]i buffering.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1431, 11 January 2012, Pages 1–12
نویسندگان
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