کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4327048 | 1614118 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Changes in pro-oxidant and antioxidant enzyme levels during cerebral hypoperfusion in rats
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کلمات کلیدی
PBSSDSPBS-TMnSODnNOSMCAO2VOAβCBFeNOSCOX-2RNSiNOSROS - ROScarotid artery occlusion - انسداد شریان کاروتیدIschemia - ایسکمیAlzheimer's disease - بیماری آلزایمرcerebral blood flow - جریان خون مغزیRoom temperature - دمای اتاقsodium dodecyl sulfate - سدیم دودسیل سولفاتinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییendothelial nitric oxide synthase - سنتاز اکسید نیتریک اندوتلیالneuronal nitric oxide synthase - سنتاز اکسید نیتریک عصبیmanganese superoxide dismutase - سوپر اکسید دیسموتاز منگنزCyclooxygenase-2 - سیکلوکوکسیژناز2Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNitrocellulose - نیترو سلولزnitric oxide synthase - نیتریک اکسید سنتازChronic cerebral hypoperfusion - هیپرفرفی عروق کرونرβ-amyloid protein - پروتئین β-آمیلوئیدreactive nitrogen species - گونه های واکنش پذیر نیتروژنReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chronic cerebral hypoperfusion is a mild ischemic condition associated with a cognitive decline which is prevalent during senescence or Alzheimer's disease. Its experimental animal model compromises permanent occlusion of the common carotid arteries (2VO) in rats, which results in neuronal damage and microglia activation. Various mechanisms, including oxidative stress, have been proposed to be involved in this process. Accordingly, we set out to characterize the changes induced in the expressions of several pro-oxidant and antioxidant enzymes in cerebral hypoperfusion. Male Wistar rats were exposed to 2VO (n = 30) or sham operation (n = 33), while a third group served as absolute control (naive, n = 16). Tissue samples from the hippocampus and frontal cortex were taken 1 and 3 days, 1 and 2 weeks and 3, 6 and 12 months following surgery. Western blot analysis was applied to determine the expressions of cyclooxygenase-2 (COX-2), endothelial, neuronal and inducible nitric oxide synthase (eNOS, nNOS and iNOS, respectively) and manganese superoxide dismutase (MnSOD). During the early phase of hypoperfusion, the COX-2 and eNOS enzyme levels increased in both the hippocampus and the frontal cortex, indicating the presence of excitotoxicity and vascular reactions caused by ischemia, while the expressions of nNOS, iNOS and MnSOD were less affected. There were significant reductions in most of the investigated enzyme levels 2 weeks and 3 months after 2VO induction, which may be a sign of neuronal loss. One year following 2VO onset, the eNOS expression was upregulated, which may strengthen the adaptation of the brain to cerebral ischemia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1321, 19 March 2010, Pages 13-19
Journal: Brain Research - Volume 1321, 19 March 2010, Pages 13-19
نویسندگان
Ãva Mracskó, Marietta Hugyecz, Ádám Institóris, Eszter Farkas, Ferenc Bari,