کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4327599 | 1614137 | 2009 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Putative antinociceptive action of nitric oxide in the caudal part of the spinal trigeminal nucleus during chronic carrageenan-induced arthritis in the rat temporomandibular joint
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کلمات کلیدی
GAPDHeNOSi.p.CFAiNOSnNOSNOSTMJKPBSPP2AFos-like immunoreactiveRT-PCRcGMP7-NIBH4tetrahydrobiopterinNG-nitro-l-arginine methyl esterSpt5potassium phosphate buffered salineDABPBSL-N6-(1-iminoethyl)-lysineLPSSp5CEGTACUFTMDstemporomandibular jointsmRNAPP1flavin adenine dinucleotide3,3′-diaminobenzidine - 3،3'-diaminobenzidine7-nitroindazole - 7-نیتریدازولcomplete Freund's adjuvant - adjuvant دوست کاملFos-LI - FOS-LIH&E - H & El-NAME - L-NAMEl-NIL - L-NILmessenger RNA - RNA messengerArthritis - آرتروزTemporomandibular disorders - اختلالات تمپوروماندیبولارTemporal bone - استخوان گیجگاهیFAD - بدintra peritoneal - داخل صفاقیCNS - دستگاه عصبی مرکزیarticular disc - دیسک مفصلیrubrospinal tract - رگروزپینالRust - زنگCAM - ساخت به کمک کامپیوترinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییendothelial nitric oxide synthase - سنتاز اکسید نیتریک اندوتلیالneuronal nitric oxide synthase - سنتاز اکسید نیتریک عصبیCIA - سیاcentral nervous system - سیستم عصبی مرکزیphosphate buffer saline - فسفات بافر شورlipopolysaccharide - لیپوپلی ساکاریدtemporomandibular joint - مفصل گیجگاهیcyclic guanosine monophosphate - مونوفسفات گوانوزین چرخه ایNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازspinal trigeminal nucleus - هسته سه گانه نخاعیHematoxylin and Eosin - هماتوکسیلین و ائوزینreverse-transcription polymerase chain reaction - واکنش زنجیره پلیمراز معکوس رونویسیprotein phosphatase 1 - پروتئین فسفاتاز 1protein phosphatase 2A - پروتئین فسفاتاز 2AMandibular condyle - کاسه ماندبولارCalmodulin - کالمودولینglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Putative antinociceptive action of nitric oxide in the caudal part of the spinal trigeminal nucleus during chronic carrageenan-induced arthritis in the rat temporomandibular joint Putative antinociceptive action of nitric oxide in the caudal part of the spinal trigeminal nucleus during chronic carrageenan-induced arthritis in the rat temporomandibular joint](/preview/png/4327599.png)
چکیده انگلیسی
In order to investigate a putative role for nitric oxide (NO) in the central nociceptive processing following carrageenan-induced arthritis in the rat temporomandibular joint (TMJ), we analyzed the immunoreactivity, gene expression and activity of nitric oxide synthases (NOS) in the caudal part of the spinal trigeminal nucleus (Sp5C) during the acute (24 h), chronic (15 days) and chronic-active (14 days-24 h) arthritis. In addition, evaluation of head-withdrawal threshold was carried out in all phases of arthritis under chronic inhibition of nNOS with the selective inhibitor 7-nitroindazole (7-NI). Neurons with nNOS-like immunoreactivity (nNOS-LI) were concentrated mainly in the lamina II of the Sp5C, showing no significant statistical difference during arthritis. Only a discrete percentage of nNOS-LI neurons expressed Fos immunoreactivity. The mRNA expression for both nNOS and endothelial nitric oxide synthases (eNOS) presented no noticeable differences among the groups. No expression of inducible nitric oxide synthase (iNOS) was detected in the Sp5C by either immunohistochemistry or reverse-transcription polymerase chain reaction (RT-PCR). Ca2+-dependent NOS activity in the ipsilateral Sp5C was significantly higher (108.3 ± 49.2%; P < 0.01) in animals during the chronic arthritis. Interestingly, this increased activity was completely abolished 24 h later, in the chronic-active arthritis. Finally, head-withdrawal threshold decreased significantly in the chronic arthritis in animals under 7-NI chronic inhibition. In conclusion, nNOS immunoreactivity and mRNA expression are stable in the Sp5C during TMJ arthritis evolution, but its activity significantly increases in the chronic-phases supporting an antinociceptive role of the nNOS as evidenced by pain threshold experiment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1302, 20 November 2009, Pages 85-96
Journal: Brain Research - Volume 1302, 20 November 2009, Pages 85-96
نویسندگان
Silvia A. Tesser-ViscaÃno, Alexandre Denadai-Souza, Simone A. Teixeira, EdÃlson Ervolino, Roelf J. Cruz-Rizzolo, Soraia K. Costa, Marcelo N. Muscará, Cláudio A. Casatti,