کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4328737 | 1614185 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transplantation of olfactory ensheathing cells enhances peripheral nerve regeneration after microsurgical nerve repair
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کلمات کلیدی
PBSDMEMSFIGFPOECseGFPCAPSCSSTOFDAPI4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoRegeneration - باززاییOlfactory Ensheathing Cells - سلول های Ensheating عصارهperipheral nervous system - سیستم عصبی پیرامونیSciatic functional index - شاخص عملکرد سیاتیکPhosphate buffered saline - فسفات بافر شورneurofilament - نوروفیلامنتgreen fluorescent protein - پروتئین فلورسنت سبزenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استTransplantation - پیوند
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
While axonal regeneration is more successful in peripheral nerve than in the central nervous system, it is by no means complete and research to enhance peripheral nerve regeneration is clinically important. Olfactory ensheathing cells (OECs) are known to enhance axonal regeneration and to produce myelin after transplantation. In contrast to Schwann cells their migratory potential and ability to penetrate glial scars is higher. This study evaluated the effect of OEC transplantation on microsurgically repaired sciatic nerves. Rat sciatic nerves were transected followed by microsurgical repair and transplantation of OECs or injection of medium without cells. Twenty-one days later the nerves were removed and prepared for either histology or electrophysiological analysis. Footprint analysis was carried out at 7, 14 and 21Â days. The OECs survived and integrated into the repaired nerves as indicated by eGFP-expressing cells aligned with neurofilament identified axons bridging the repair site. Moreover, regenerated axons were myelinated by the transplanted OECs and nodes of Ranvier were formed. Conduction velocity in the OEC transplant group was increased in comparison to the microsurgical repair alone, and improved stepping was observed in the transplant group. These results suggest that presentation of OECs at the time of nerve injury enhances regeneration and improves functional outcome. Even a modest improvement in nerve regeneration could have significant clinical implications for reconstructive nerve surgery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1254, 13 February 2009, Pages 10-17
Journal: Brain Research - Volume 1254, 13 February 2009, Pages 10-17
نویسندگان
Christine Radtke, Ayal A. Aizer, Samuel K. Agulian, Karen L. Lankford, Peter M. Vogt, Jeffery D. Kocsis,