کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4329651 1614229 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of GABAA receptor α1 subunit mRNA and protein in rat neocortex following photothrombotic infarction
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Expression of GABAA receptor α1 subunit mRNA and protein in rat neocortex following photothrombotic infarction
چکیده انگلیسی

Photothrombotic infarcts of the neocortex result in structural and functional alterations of cortical networks, including decreased GABAergic inhibition, and can generate epileptic seizures within 1 month of lesioning. In our study, we assessed the involvement and potential changes of cortical GABAA receptor (GABAAR) α1 subunits at 1, 3, 7, and 30 days after photothrombosis. Quantitative competitive reverse transcription-polymerase chain reaction (cRT-PCR) and semi-quantitative Western blot analysis were used to investigate GABAAR α1 subunit mRNA and protein levels in proximal and distal regions of perilesional cortex and in homotopic areas of young adult Sprague-Dawley rats. GABAAR α1 subunit mRNA levels were decreased ipsilateral and contralateral to the infarct at 7 days, but were increased bilaterally at 30 days. GABAAR α1 subunit protein levels revealed no significant change in neocortical areas of both hemispheres of lesioned animals compared with protein levels of sham-operated controls at 1, 3, 7, and 30 days. At 30 days, GABAAR α1 subunit protein expression was significantly increased in lesioned animals within proximal and distal regions of perilesional cortex compared with distal neocortical areas contralaterally (Student's t-test, p < 0.05). Short- and long-term alterations of mRNA and protein levels of the GABAAR α1 subunit ipsilateral and contralateral to the lesion may influence alterations in cell surface receptor subtype expression and GABAAR function following ischemic infarction and may be associated with formative mechanisms of poststroke epileptogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1210, 19 May 2008, Pages 29–38
نویسندگان
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