کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4330537 1614269 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An investigation into the lipid-binding properties of α-, β- and γ-synucleins in human brain and cerebrospinal fluid
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
An investigation into the lipid-binding properties of α-, β- and γ-synucleins in human brain and cerebrospinal fluid
چکیده انگلیسی

Parkinson's disease (PD) and dementia with Lewy bodies (DLB) are both characterized by the formation and intraneuronal accumulation of fibrillar aggregates of α-synuclein (α-syn) protein in affected brain regions. α-Syn has biochemical properties and a structural motif characteristic of fatty acid binding proteins. Using the fatty acid binding resin Lipidex-1000, we investigated the capture of α-, β-, and γ-syn proteins as lipid-associated proteins from normal and DLB brain lysates, and from normal human cerebrospinal fluid (CSF). These were eluted from Lipidex-1000 and analyzed by SDS–NuPAGE followed by Western blotting. Using this methodology, we have been able to extract full-length and truncated forms of α-syn from brain lysates. We also extracted low levels of β-syn from DLB brains, but failed to extract any γ-syn. We were able to capture only full-length monomeric α-syn from normal human CSF. Our data confirm the fatty acid binding properties of α-syn, and to a lesser extent β-syn, but suggest that γ-syn does not share this same characteristic.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1170, 19 September 2007, Pages 103–111
نویسندگان
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