کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4331383 1614298 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modulation of alpha and beta crystallin expression in rat retinas with ocular hypertension-induced ganglion cell degeneration
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Modulation of alpha and beta crystallin expression in rat retinas with ocular hypertension-induced ganglion cell degeneration
چکیده انگلیسی

The expression of alpha (αA and αB) and beta (βA1/A3, βA2, βA4, and βB2) crystallin genes were analyzed at the mRNA and protein levels in rat retinas with ocular hypertension-induced ganglion cell death. An animal model with progressive loss of retinal ganglion cells (RGC) was generated by elevation of intraocular pressure (IOP). The estimated RGC loss was approximately 8% and 20% at 2 and 5 weeks post IOP elevation, respectively. mRNA and protein quantification showed that alpha and beta crystallin genes were downregulated at both transcriptional (αA, αB, βA1/A3, βA4, and βB2 approximately 50% and βA2 ~ 40%) and protein (αA ~ 50%, αB ~ 63%, βA1/A3 ~ 70%, and βB2 ~ 38%) levels 2 weeks after IOP elevation. In experimental retinas 5 weeks after IOP elevation, the levels of crystallin mRNAs were higher than at 2 weeks and were comparable to that of control retinas. However, the levels of the corresponding proteins were still lower (αA, αB, and βB2 approximately 37% and βA1/A3 ~ 70%) than in control retinas. Furthermore, we found that the expression of these genes in the retina is predominantly localized to the cells in the GCL and to a lesser degree in the INL and ONL. Colocalization of the crystallin-positive and Fluorogold retrogradely labeled cells indicated that the cells expressing alpha and beta crystallins in the GCL are RGCs. In summary, we showed that alpha and beta crystallins are expressed in the retina predominantly by RGCs and that their expression is affected by ocular hypertension.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1141, 13 April 2007, Pages 1–9
نویسندگان
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