Sex-specific densities of estrogen receptors alpha and beta in the subnuclei of the nucleus tractus solitarius, hypoglossal nucleus and dorsal vagal motor nucleus weanling rats

In rats ventilatory responses to N-methyl-d-aspartate (NMDA) receptor modulation are sexually dimorphic and may be altered by manipulating brain levels of estrogen receptors. Here we used image analysis and immunohistochemistry in weanling male and female rats to quantitate areas and densities of ER alpha and ER beta-positive neurons within medullary regions associated with cardiopulmonary regulation including the hypoglossal nucleus, subnuclei of the nucleus of the solitary tract (NTS), and the dorsal motor nucleus of the vagus. Weanling rats were selected because ventilation, metabolic rate, and body and brain weights are comparable at this age and there are no large fluctuations in plasma hormone levels. Females, relative to males, had smaller areas in the A2 region and parts of the NTS. Counts and densities for ER alpha were greater in females than males in almost all regions studied. In contrast sex differences in ER beta were found in fewer nuclei, but in those higher counts and densities were noted in females. In general, ER beta-positive neurons in the brainstem regions examined were less prevalent than ER alpha neurons. Thus, in weanling rats sex affected ER alpha and ER beta neuronal densities in brainstem regions associated with cardiopulmonary regulation that may be responsible for sex differences in control of breathing.