کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4336445 | 1295213 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the prion protein 3F4 epitope and its use as a molecular tag
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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![عکس صفحه اول مقاله: Characterization of the prion protein 3F4 epitope and its use as a molecular tag Characterization of the prion protein 3F4 epitope and its use as a molecular tag](/preview/png/4336445.png)
چکیده انگلیسی
These and other observations have led to the commonly accepted notion that the 3F4 epitope consists of the tetra-peptide Met-Lys-His-Met. In this study, we have identified the minimal epitope for 3F4 by studying its binding to synthetic peptides and by analysis of mutated ovine PrP::GFP constructs expressed in cell culture. We have found that the 3F4 epitope consists of a hepta-peptide (Lys-Thr-Asn-Met-Lys-His-Met), which in sheep encompass residues 109-115. We found that Lys 109 is critically important for 3F4 binding, as omission, or substitution of this residue to Ala resulted in no binding. We also demonstrate that the hepta-peptide constituting the minimal 3F4 epitope, can be used as a discrete, moveable high-affinity molecular tag. Thus, the 3F4 antibody can find its use beyond prion research.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 165, Issue 2, 30 September 2007, Pages 183-190
Journal: Journal of Neuroscience Methods - Volume 165, Issue 2, 30 September 2007, Pages 183-190
نویسندگان
Christoffer Lund, Christel Moræus Olsen, Heidi Tveit, Michael A. Tranulis,