کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4336630 | 1295220 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A more efficient enzyme-linked immunosorbent assay for measurement of α-synuclein in cerebrospinal fluid
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
We describe a modification of a previously described assay for the quantification of α-synuclein in naive cerebrospinal fluid, which allows for a more efficient quantification of α-synuclein. Detection limit of the assay is 3.8 ng/ml and the assay is linear until 300 ng/ml. Inter-assay and intra-assay coefficients of variation are below 15% in a wide range of concentrations. Mean recovery of the assay is 94%. The 95% upper limit of the reference range (p95) in a group of neurological controls above the age of 45 years is 62 ng/ml. This assay can be routinely applied for quantification of α-synuclein in cerebrospinal fluid, but not in serum, and this may serve as a possible biomarker for α-synucleinopathies such as Parkinson's disease and multiple system atrophy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 168, Issue 1, 15 February 2008, Pages 182-185
Journal: Journal of Neuroscience Methods - Volume 168, Issue 1, 15 February 2008, Pages 182-185
نویسندگان
Wieneke J.A. van Geel, W. Farid Abdo, René Melis, Sonja Williams, Bastiaan R. Bloem, Marcel M. Verbeek,