کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4337605 1614806 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Prenatal betamethasone does not affect glutamatergic or GABAergic neurogenesis in preterm newborns
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Prenatal betamethasone does not affect glutamatergic or GABAergic neurogenesis in preterm newborns
چکیده انگلیسی


• Prenatal betamethasone does not alter glutamatergic neurogenesis at birth or postnatal d3.
• Prenatal betamethasone does not affect GABAergic neurogenesis at birth or postnatal d3.
• Prenatal betamethasone does not change GABA+ population in ganglionic eminence.
• Prenatal betamethasone does not cause apoptosis of neuronal progenitors.

Prenatal glucocorticoids (GCs) are routinely used for pregnant women in preterm labor to prevent respiratory distress syndrome and intraventricular hemorrhage in premature infants. However, the effect of antenatal GCs on neurogenesis in preterm neonates remains elusive. Herein, we hypothesized that prenatal GCs might suppress both glutamatergic and GABAergic neurogenesis in preterm rabbits and that this treatment would induce distinct changes in the expression of transcription factors regulating these developmental events. To test our hypotheses, we treated pregnant rabbits with betamethasone at E27 and E28, delivered the pups at E29 (term = 32 d), and assessed neurogenesis at birth and postnatal day 3. We quantified radial glia (Sox2+) and intermediate progenitor cells (Tbr2+) in the dorsal cortical subventricular zone to assess glutamatergic neuronal progenitors, and counted Nkx2.1+ and Dlx2+ cells in the ganglionic eminence to evaluate GABAergic neurogenesis. In addition, we assayed transcription factors regulating neurogenesis. We found that prenatal GCs did not affect the densities of radial glia and intermediate progenitors of glutamatergic or GABAergic neurons. The number of GABA+ interneurons in the ganglionic eminence was similar between the prenatal GC-treated pups compared to untreated controls. Moreover, the mRNA expression of transcription factors, including Pax6, Ngn1/2, Emx1/2, Insm1, Dlx1, Nkx2.1, and Gsh2, were comparable between the two groups. However, there was a transient elevation in Mash1 protein in betamethasone-treated pups relative to controls at birth. These data suggest that prenatal GC treatment does not significantly impact the balance of glutamatergic and GABAergic neurogenesis in premature infants.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 270, 13 June 2014, Pages 148–157
نویسندگان
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