Low-dose GYKI-52466: Prophylactic preconditioning confers long-term neuroprotection and functional recovery following hypoxic-ischaemic brain injury

► We evaluated GYKI-52466 in a rat model of hypoxic-ischaemic brain injury. ► GYKI-52466 is neuroprotective at very low doses. ► GYKI-52466 is neuroprotective at very long pre-administration intervals. ► GYKI-52466 preconditioning likely involves metabotropic mechanisms.