Synaptic dynamics and long-term plasticity at synapses of Purkinje cells onto neighboring Purkinje cells of a mormyrid fish: A dual cell recording study

The input synapses of cerebellar Purkinje cells (PCs) have been extensively studied and much has been learned about their dynamics, plasticity and functionality. In contrast there is limited information available about PC output synapses. This study uses dual cell recording methods to investigate synaptic dynamics and plasticity at individual PC synapses onto neighboring PCs in in vitro preparations of the mormyrid cerebellum. This synaptic connectivity may be strong or weak. For strong connections, inhibitory postsynaptic potentials (IPSPs) or currents (IPSCs) are synchronized with the action potentials of the presynaptic cell. For weak connections, however, the pre- and postsynaptic potentials are no longer synchronized, and presynaptic burst firing at intraburst rates of ∼50 Hz or higher is required to reliably induce the postsynaptic inhibition. A depression of this postsynaptic inhibition was observed for both types of connectivity following repeated presynaptic bursts, which was subsequently largely reversed following pairings of the presynaptic burst-induced IPSPs/IPSCs with evoked burst firing of the postsynaptic cell. Moreover, the original postsynaptic depression was found to be either augmented or reversed depending on the temporal order of each pair of additional pre- and postsynaptic cell activations, hence demonstrating a reversible and spike timing-dependent plasticity (STDP) at this synapse.

► Dendritic and axonal spikes of mormyrid Purkinje cells are functionally distinct.
► Synaptic inhibition of PCs can be synchronized or unsynchronized with presynaptic spiking.
► PC output synapses can express a long-term, reversible and spike timing-dependent plasticity.