کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4338836 1614889 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interleukin-1β causes long-term potentiation deficiency in a mouse model of septic encephalopathy
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Interleukin-1β causes long-term potentiation deficiency in a mouse model of septic encephalopathy
چکیده انگلیسی

Sepsis induces multiple organ dysfunction syndrome including septic encephalopathy (SE), which results in cognitive impairment. However, an effective treatment for SE remains unknown. We determined the role of interleukin-1β (IL-1β) in long-term potentiation (LTP) deficiency after SE. At first, endotoxin level in the blood was increased at 24 h after cecum ligation and puncture (CLP) (i.e. SE model). Second, the expression of IL-1β and its receptor in the hippocampus was determined by immunohistochemistry and immunoblotting. The number of Iba1-positive cells and their expression of IL-1β were enhanced by CLP with disruption of the blood brain barrier. Also, Iba1, IL-1β, and occludin protein expressions were consistent with immunohistochemical results. Third, we used an electrophysiological technique and observed the LTP deficiency, a hallmark of learning and memory, in the slices of hippocampus after CLP. Since type 1 interleukin-1 receptors (IL-1R1s) on neuronal cells were increased in the hippocampus, we utilized IL-1R1 antagonist. Pre-incubation with IL-1R1 antagonist for 30 min before recording of field excitatory post-synaptic potentials (fEPSPs) in the hippocampus canceled LTP deficiency after CLP. These results suggest the novel importance of IL-1β in synaptic plasticity deficiency associated with sepsis-induced brain inflammation. In a mouse model of SE, IL-1R1 inhibition is important in protecting synaptic function of the hippocampus after induction of SE.

▶IL-1β is mainly increased in Iba-1 positive cells and type-1 IL-1 receptor (IL-1R1) is increased in neurons in hippocampus of CLP mice. ▶IL-1R1 is critical for the deficiency of LTP, a hallmark of spatial memory in mice after CLP. ▶These data indicate that IL-1 antagonist may alleviate cognitive deficiency after SE.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 187, 28 July 2011, Pages 63–69
نویسندگان
, , , , , , ,