کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4339080 1614898 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Calpain-mediated regulation of stargazin in adult rat brain
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Calpain-mediated regulation of stargazin in adult rat brain
چکیده انگلیسی

Changes in AMPA receptors have been proposed to underlie changes in synaptic efficacy in hippocampus and other brain structures. Calpain activation has also been discussed as a potential mechanism to produce lasting modifications of synaptic structure and function. Stargazin is a member of the family of transmembrane AMPA receptor associated proteins (TARPs), which participates in trafficking of AMPA receptors and regulates their kinetic properties. We report here that preincubation of thin (20 μm) frozen rat brain sections with calcium changes the immunological properties of stargazin, an effect totally blocked by a calpain inhibitor. Immunocytochemistry indicates that in situ calpain activation produces a decreased immunoreactivity for stargazin in the neuropil throughout the brain, and Western blots confirmed that a similar treatment decreased stargazin levels. Interestingly, the same treatment did not modify the immunoreactivity for another TARP member, γ-8, although it increased immunoreactivity in cell bodies in hippocampus, an effect that was not blocked by calpain inhibition. These results strongly suggest the involvement of calpain in the regulation of AMPA receptor targeting and function through truncation of stargazin.

Research highlights▶Calpain activation results in rapid down-regulation of stargazin but not TARP-γ-8. ▶This is due to calpain-mediated truncation of the C-terminal domain of stargazin. ▶In contrast, TARP-γ-8 is not truncated by calpain. ▶This could account for activity-dependent regulation of synaptic AMPA receptors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 178, 31 March 2011, Pages 13–20
نویسندگان
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