کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4340073 | 1295782 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neuronal depolarization modifies motor protein mobility
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کلمات کلیدی
DICDHCmRFPMTOCDynein heavy chainDynein intermediate chainTransport - حمل و نقل یا ترابریdynein - دینینDIV - دیوdays in vitro - روز in vitroMicrotubule - ریزلوله یا میکروتوبولNeuronal activity - فعالیت عصبیmicrotubule-organizing center - مرکز میکروتوبولی سازماندهیNeuron - نورونmonomeric red fluorescent protein - پروتئین فلورسنت قرمز مونومرMotor protein - پروتئین موتور
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Active neuronal transport along microtubules participates in the targeting of mRNAs, proteins and organelles to their sites of action. Cytoplasmic dynein represents a minus-end-directed microtubule-dependent motor protein. Due to the polarity of microtubules in axonal and distal dendritic compartments, with microtubule minus-ends pointing toward the inside of the cell, dyneins mainly mediate retrograde transport pathways in neurons. Since dyneins transport synaptic proteins, we asked whether changes in neuronal activity would in general influence dynein transport. KCl-induced depolarization, a condition that mimics the effects of neuronal activity, or pharmacological blockade of neuronal action potentials, respectively, was combined with neuronal live cell imaging, using an autofluorescent dynein intermediate chain fusion (monomeric red fluorescent protein [mRFP]-dynein intermediate chain [DIC]) as a model protein. Notably, we found that induced activity significantly reduced dynein particle mobility, as well as both the total distance and velocity of movements in mouse cultured hippocampal neurons. In contrast, blockade of neuronal action potentials through TTX did not alter any of the parameters analyzed. Neuronal depolarization processes therefore represent candidate mechanisms to regulate intracellular transport of neuronal cargoes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 160, Issue 1, 21 April 2009, Pages 1-5
Journal: Neuroscience - Volume 160, Issue 1, 21 April 2009, Pages 1-5
نویسندگان
K. Lardong, C. Maas, M. Kneussel,