کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341004 | 1295818 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glycine receptors are functionally expressed on bullfrog retinal cone photoreceptors
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کلمات کلیدی
DCKAOPLONLINLEC50ECLGlyRIPL5,7-dichlorokynurenic acid - 5،7-dichlorokynurenic اسیدDMSO - DMSOphosphate buffer - بافر فسفاتimmunofluorescence double labeling - برچسب ایمنی فلوئورسانسDimethyl sulfoxide - دیمتیل سولفواکسیدRetina - شبکیهwhole cell recording - ضبط کامل سلولouter plexiform layer - لایه بیرونی بیرونیouter nuclear layer - لایه بیرونی هسته ایinner plexiform layer - لایه داخلی لایهinner nuclear layer - لایه داخلی هسته ایMolecular weight - وزن مولکولیreversal potential - پتانسیل معکوسcone photoreceptor - گیرنده نور مخروطیglycine receptor - گیرنده گلیسینErev - یورو
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Using immunocytochemical and whole cell recording techniques, we examined expression of glycine receptors on bullfrog retinal cone photoreceptors. Immunofluorescence double labeling experiments conducted on retinal sections and isolated cell preparations showed that terminals and inner segments of cones were immunoreactive to both α1 and β subunits of glycine receptors. Moreover, application of glycine induced a sustained inward current from isolated cones, which increased in amplitude in a dose-dependent manner, with an EC50 (concentration of glycine producing half-maximal response) of 67.3±4.9 μM, and the current was blocked by the glycine receptor antagonist strychnine, but not 5,7-dichlorokynurenic acid (DCKA) of 200 μM, a blocker of the glycine recognition site at the N-methyl-d-aspartate (NMDA) receptor. The glycine-induced current reversed in polarity at a potential close to the calculated chloride equilibrium potential, and the reversal potential was changed as a function of the extracellular chloride concentration. These results suggest that strychnine-sensitive glycine receptors are functionally expressed in bullfrog cones, which may mediate signal feedback from glycinergic interplexiform cells to cones in the outer retina.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 146, Issue 1, 25 April 2007, Pages 427-434
Journal: Neuroscience - Volume 146, Issue 1, 25 April 2007, Pages 427-434
نویسندگان
L.-H. Ge, S.-C. Lee, J. Liu, X.-L. Yang,