کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341095 | 1295822 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microarray profile of seizure damage-refractory hippocampal CA3 in a mouse model of epileptic preconditioning
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کلمات کلیدی
KIF1ASTARD4GAPDHRTPCRNeuNkainic acid - اسید کرییکElectroencephalogram - الکتروانسفالوگرافیEpilepsy - بیماری صرعTranscriptome - ترانسکریپتومTolerance - تلرانس TUNEL - تونلApoptosis - خزان یاختهایKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوNeuroprotection - محافظت نورونی یا محافظت از عصبEEG - نوار مغزیpolymerase chain reaction - واکنش زنجیره ای پلیمرازreal-time quantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استPCR - واکنش زنجیرهٔ پلیمرازBok - کتابglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A neuroprotected state can be acquired by preconditioning brain with a stimulus that is subthreshold for damage (tolerance). Acquisition of tolerance involves coordinate, bi-directional changes to gene expression levels and the re-programmed phenotype is determined by the preconditioning stimulus. While best studied in ischemic brain there is evidence brief seizures can confer tolerance against prolonged seizures (status epilepticus). Presently, we developed a model of epileptic preconditioning in mice and used microarrays to gain insight into the transcriptional phenotype within the target hippocampus at the time tolerance had been acquired. Epileptic tolerance was induced by an episode of non-damaging seizures in adult C57Bl/6 mice using a systemic injection of kainic acid. Neuron and DNA damage-positive cell counts 24 h after status epilepticus induced by intraamygdala microinjection of kainic acid revealed preconditioning given 24 h prior reduced CA3 neuronal death by â¼45% compared with non-tolerant seizure mice. Microarray analysis of over 39,000 transcripts (Affymetrix 430 2.0 chip) from microdissected CA3 subfields was undertaken at the point at which tolerance was acquired. Results revealed a unique profile of small numbers of equivalently up- and down-regulated genes with biological functions that included transport and localization, ubiquitin metabolism, apoptosis and cell cycle control. Select microarray findings were validated post hoc by real-time polymerase chain reaction and Western blotting. The present study defines a paradigm for inducing epileptic preconditioning in mice and first insight into the global transcriptome of the seizure-damage refractory brain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 150, Issue 2, 5 December 2007, Pages 467-477
Journal: Neuroscience - Volume 150, Issue 2, 5 December 2007, Pages 467-477
نویسندگان
S. Hatazaki, C. Bellver-Estelles, E.M. Jimenez-Mateos, R. Meller, C. Bonner, N. Murphy, S. Matsushima, W. Taki, J.H.M. Prehn, R.P. Simon, D.C. Henshall,