کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341300 | 1295830 | 2007 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microglia and macrophages express tumor necrosis factor receptor p75 following middle cerebral artery occlusion in mice
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کلمات کلیدی
TBSTNFTNF-RHPRT1pMCAOFCSPFAHRPSSCGFPDABMCAPBSAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییPermanent middle cerebral artery occlusion - انسداد شریان مغزی میانی دائمیFocal cerebral ischemia - ایسکمی مغز مرکزی کانونیTris-buffered saline - تریس بافر شورRoom temperature - دمای اتاقdiaminobenzidine - دیامینو بنزیدینfetal calf serum - سرم گوساله جنینCytokines - سیتوکین هاSignaling - سیگنالینگmiddle cerebral artery - شریان مغزی میانیtumor necrosis factor - فاکتور نکروز تومورPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریbone marrow - مغز استخوانknockout - ناکاوتHypoxanthine phosphoribosyltransferase 1 - هیپوکسانتین فسفریبوسیل ترانسفراز 1polymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازparaformaldehyde - پارافرمالدهیدHorseradish peroxidase - پراکسیداز هوررادیشgreen fluorescent protein - پروتئین فلورسنت سبزReceptors - گیرنده هاTumor necrosis factor receptors - گیرنده های فاکتور نکروز تومور
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The proinflammatory and potential neurotoxic cytokine tumor necrosis factor (TNF) is produced by activated CNS resident microglia and infiltrating blood-borne macrophages in infarct and peri-infarct areas following induction of focal cerebral ischemia. Here, we investigated the expression of the TNF receptors, TNF-p55R and TNF-p75R, from 1 to 10 days following permanent occlusion of the middle cerebral artery in mice. Using quantitative polymerase chain reaction (PCR), we observed that the relative level of TNF-p55R mRNA was significantly increased at 1-2 days and TNF-p75R mRNA was significantly increased at 1-10 days following arterial occlusion, reaching peak values at 5 days, when microglial-macrophage CD11b mRNA expression was also increased. In comparison, the relative level of TNF mRNA was significantly increased from 1 to 5 days, with peak levels 1 day after arterial occlusion. In situ hybridization revealed mRNA expression of both receptors in predominantly microglial- and macrophage-like cells in the peri-infarct and subsequently in the infarct, and being most marked from 1 to 5 days. Using green fluorescent protein-bone marrow chimeric mice, we confirmed that TNF-p75R was expressed in resident microglia and blood-borne macrophages located in the peri-infarct and infarct 1 and 5 days after arterial occlusion, which was supported by Western blotting. The data show that increased expression of the TNF-p75 receptor following induction of focal cerebral ischemia in mice can be attributed to expression in activated microglial cells and blood-borne macrophages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 144, Issue 3, 9 February 2007, Pages 934-949
Journal: Neuroscience - Volume 144, Issue 3, 9 February 2007, Pages 934-949
نویسندگان
K.L. Lambertsen, B.H. Clausen, C. Fenger, H. Wulf, T. Owens, F. Dagnaes-Hansen, M. Meldgaard, B. Finsen,