کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4342727 | 1295888 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Focal cerebral ischemia/reperfusion injury in mice induces hematopoietic prostaglandin D synthase in microglia and macrophages
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSGST-πpgdsH-PGDSIBA1MAP-2MCAOPGD2eGFPGFAPMCACOXcyclooxygenase - آنزیم سیکلواکسیژنازinflammation - التهاب( توروم) middle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیTissue repair - تعمیر بافتIschemic stroke - سکته مغزی ایسکمیکmiddle cerebral artery - شریان مغزی میانیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریionized calcium-binding adapter molecule 1 - ملکول آداپتور اتصال دهنده کلسیم یونیزه 1Microglia/macrophage - میکروگلای / ماکروفاژhematopoietic prostaglandin D synthase - هموگلوبین پروستاگلاندین D سنتازGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استMicrotubule associated protein 2 - پروتئین مرتبط با میکروتوبول 2Prostaglandin D synthase - پروستاگلاندین D سنتازProstaglandin D2 - پروستاگلاندین D2prostaglandin - پروستاگلاندینها
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hematopoietic prostaglandin D synthase is a key enzyme in synthesis of prostaglandin D. Hematopoietic prostaglandin D synthase is expressed in microglia of the developing mouse brain. This study determined the serial changes and cellular localization of hematopoietic prostaglandin D synthase, and its role in cerebral ischemia/reperfusion injury using C57BL/6 mice (n=84) and bone marrow chimera mice (n=16). The latter mice were selected based on their expression of enhanced green fluorescent protein in bone marrow/blood-derived monocytes/macrophages. The middle cerebral artery was occluded for 60 min, followed by reperfusion. Hematopoietic prostaglandin D synthase expression was examined by immunohistochemistry and Western blotting. Hematopoietic prostaglandin D synthase-positive cells were mainly expressed in the peri-ischemic area at 12 h (P<0.05) and 24 h (P<0.001) after reperfusion, while they were mostly found in the transition area at 48-72 h postreperfusion (P<0.001). There was a significant increase in staining intensity as well as number of hematopoietic prostaglandin D synthase-positive cells in the ischemic core at 5-7 (P<0.001) days postreperfusion. Hematopoietic prostaglandin D synthase-positive cells also co-expressed ionized calcium-binding adapter molecule 1, a marker of microglia/macrophages, and cyclooxygenase-2, but not markers of neurons, oligodendrocytes and astrocytes. Until 72 h postreperfusion, many enhanced green fluorescent protein-positive cells were negative for hematopoietic prostaglandin D synthase, but the number of hematopoietic prostaglandin D synthase-enhanced green fluorescent protein coexpressing cells increased significantly at 5-7 days after reperfusion. Our results indicate that hematopoietic prostaglandin D synthase is mainly produced by endogenous microglia until 72 h after reperfusion, but at 7 days after reperfusion, it is also produced by migrating bone marrow/blood-derived macrophages in the ischemic brain tissue. We speculate that hematopoietic prostaglandin D synthase in the brain has different functions during early and late phases of ischemia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 145, Issue 2, 16 March 2007, Pages 520-529
Journal: Neuroscience - Volume 145, Issue 2, 16 March 2007, Pages 520-529
نویسندگان
M. Liu, N. Eguchi, Y. Yamasaki, Y. Urade, N. Hattori, T. Urabe,