کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4343911 | 1615143 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Altered expression of miR-146a in myasthenia gravis
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کلمات کلیدی
TLR4TRAF-6miRNA-146aMicroRNA-146aEAMGNF-κBPBMCsAChRNMJRheumatoid arthritis - آرتریتروماتوئیدNeuromuscular junction - اتصال عصبی عضلانیmiR-146a - به miR-146Aperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیIRAK-1 - عراق-1TNF receptor associated factor 6 - فاکتور مرتبط با گیرنده TNF 6nuclear factor-κB - فاکتور هسته ای κBB cell - لنفوسیت بیSystemic lupus erythematosus - لوپوس اریتماتوی سیستمیکSLE - لوپوس منتشر یا لوپوس اریتماتوس سیستمیکToll like receptor 4 - مانند گیرنده 4experimental autoimmune myasthenia gravis - مایواستنیا گراوی خود ایمنی تجربیMyasthenia gravis - میاستنی گراویسacetylcholine receptor - گیرنده استیل کولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The purpose of this study was to analyze the expression of miR-146a in PBMCs obtained from patients with myasthenia gravis (MG) and healthy controls and to investigate the effect of the inhibition of miR-146a on the activation of AchR specific B cells obtained from mice. The expression of miR-146a levels in PBMCs obtained from patients with MG and healthy controls were determined by qRT-PCR. MiR-146a's complementary fragment, AntagomiR-146a, was synthesized as inhibitor, and the nonfunctional fragment, which has similar construction to AntagomiR-146a, was synthesized as negative control inhibitor. The expression of miR-146a, CD40, CD80 and CD86 on AchR specific B cells were analyzed by qRT-PCR and flow cytometry. Western blotting was used to detect the expression of TLR4, NF-κB and Bcl-2 .The expression of miRNA-146a in PBMCs obtained from patients with MG was significantly upregulated compared to healthy controls (P < 0.01). Transfection with miR-146a inhibitor dramatically decreased expression of miR-146a, CD40, CD80, TLR4 and NF-κB on AchR specific B cells compared to mock transfected cells. We conclude that abnormal expression/regulation of miR-146a may play an important role in the regulation of AchR specific B cells and contribute to the pathogenesis of MG.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 555, 25 October 2013, Pages 85-90
Journal: Neuroscience Letters - Volume 555, 25 October 2013, Pages 85-90
نویسندگان
Jiayin Lu, Mei Yan, Yuzhong Wang, Junmei Zhang, Huan Yang, Fa-fa Tian, Wenbin Zhou, Ning Zhang, Jing Li,