Mesenspheres of neural crest-derived cells enriched from bone marrow stromal cell subpopulation

► The EGF/FGF2-responsive BMSC subpopulation (BMSC-C2) showed a significant sphere-forming ability. ► BMSC-C2-derived mesenspheres displayed a NCC-like phenotype. ► BMSC-C2-derived mesenspheres were self-renewable and able to differentiate toward neural lineages.