کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4346133 | 1296773 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrophysiological characteristics of identified kidney-related neurons in adult rat spinal cord slices
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Whole-cell patch-clamp recordings were made from kidney-related neurons in the intermediolateral cell column (IML) in horizontal slices of thoracolumbar spinal cord from adult rats. Kidney-related neurons were identified in vitro subsequent to inoculation of the kidney with a fluorescent, retrograde, transynaptic pseudorabies viral label (i.e., PRV-152). Kidney-related neurons detected in the IML expressed choline acetyltransferase, characteristic of spinal preganglionic motor neurons. Their mean resting potential was â51 ± 4 mV and input resistance was 448 ± 39 MΩ. Both spontaneous inhibitory and excitatory post-synaptic currents (i.e., sIPSCs and sEPSCs) were observed in all neurons. The mean frequency for sEPSCs (3.1 ± 1 Hz) was approximately 2.5 times that for sIPSCs (1.4 ± 0.3 Hz). Application of the glycine and GABAA receptor-linked Clâ channel blocker, picrotoxin (100 μM) blocked sIPSCs, while the ionotropic glutamate receptor antagonist, kynurenic acid (1 mM) blocked all sEPSCs, indicating they were mediated by GABA/glycine and glutamate receptors, respectively. Thus, using PRV-152 labeling allowed whole-cell patch-clamp recording of neurons in the adult spinal cord, which were kidney-related. Excitatory glutamatergic input dominated synaptic responses in these cells, the membrane characteristics of which resembled those of immature IML neurons. Combined PRV-152 pre-labeling and whole-cell patch-clamp recordings may allow more effective analysis of synaptic plasticity seen in adult models of injury or chronic disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 474, Issue 3, 3 May 2010, Pages 168-172
Journal: Neuroscience Letters - Volume 474, Issue 3, 3 May 2010, Pages 168-172
نویسندگان
Andrei V. Derbenev, Hanad Duale, Alexander G. Rabchevsky, Bret N. Smith,