کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4347743 | 1296858 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transactivation by Runt related factor-2 of matrix metalloproteinase-13 in astrocytes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DMEMCbfβOSE2αMEMMMP13OPNMAP2RT-PCRRUNX2GFPPBSFBSGFAPGAPDHDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccoα-minimum essential medium - α حداقل محیط ضروریAstrocytes - آستروسیتOsteopontin - استئوپنتینCNS - دستگاه عصبی مرکزیRetinoic acid - رتینوئیک اسیدfetal bovine serum - سرم جنین گاوcentral nervous system - سیستم عصبی مرکزیMatrix metalloproteinase-13 - ماتریکس متالوپروتئیناز 13Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریreverse-transcription polymerase chain reaction - واکنش زنجیره پلیمراز معکوس رونویسیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالgreen fluorescent protein - پروتئین فلورسنت سبزmicrotubule-associated protein 2 - پروتئین مرتبط با میکروتوبول 2glyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have previously shown functional expression by osteoblasts of signaling machineries required for neurotransmission in the brain. In this study, we have evaluated possible functional expression of different osseous genes in the brain. In embryonic and adult mouse brains, mRNA expression was invariably seen for the master regulator of osteoblastic differentiation Runt related factor-2 (Runx2), in addition to the partner protein core binding factor-β and their targets such as osteopontin (OPN) and matrix metalloproteinase-13 (MMP13), but not for collagen-I or osteocalcin. In pluripotent P19 progenitor cells, Runx2 mRNA expression was drastically increased along with mRNA expression of an astrocytic marker, but not with neuronal marker mRNA expression. Both mRNA and corresponding protein were detected for Runx2 in cultured rat neocortical astrocytes and astrocytic C6 glioma cells. In C6 glioma cells, transient overexpression of Runx2 significantly increased mRNA expression of MMP13, but not of OPN. Moreover, transient overexpression of Runx2 significantly increased luciferase activity in C6 glioma cells transfected with the reporter plasmid linked to a wild-type Runx2 binding element in the MMP13 promoter, but not in cells with a mutated element. These results suggest that Runx2 signal input may lead to transactivation of MMP13 gene without affecting OPN expression in astrocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 451, Issue 2, 20 February 2009, Pages 99-104
Journal: Neuroscience Letters - Volume 451, Issue 2, 20 February 2009, Pages 99-104
نویسندگان
Takeshi Takarada, Yukio Yoneda,