کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4347896 1615176 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Abnormal cleavage of APP impairs its functions in cell adhesion and migration
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Abnormal cleavage of APP impairs its functions in cell adhesion and migration
چکیده انگلیسی

Amyloid precursor protein (APP) is expressed ubiquitously but its wrong cleavage only occurs in central nervous system. In this research, overexpression of wild type human APP695 was found to stimulate the adhesion and migration of N2a cells. In the cells co-transfected by familial Alzheimer’s disease (FAD)-linked Swedish mutant of APP695 gene plus △E9 deleted presenilin1 gene (N2a/Swe.△9), however, this stimulating function was impaired compared to that in the cells co-transfected by Swedish mutant of APP695 gene plus dominant negative mutant of presenilin1 D385A gene (N2a/Swe.385). Furthermore, it was also found that the phosphorylation of FAK Tyr-861 and GSK-3β Ser-9 was reduced in N2a/Swe.Δ9 cells, which can be possibly taken as a reasonable explanation for the underlying mechanism. Our results suggest that impaired cell adhesion and migration induced by abnormal cleavage of APP could contribute to the pathological effects in FAD brain.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 450, Issue 3, 6 February 2009, Pages 327–331
نویسندگان
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