CCK-8S inhibited the NMDA-activated current of cultured hippocampal neuron under normal and ethanol exposure conditions

Whole-cell patch clamp technique was used to study the modulatory effect of Cholecystokin-8S (CCK-8S), a neuropeptide, on NMDA-activated current (INMDA) in cultured rat hippocampal neurons under condition of normal and ethanol exposure. Our study showed that CCK-8S inhibited INMDA in a non-concentration and non-competition dependent manner. Ethanol significantly inhibited INMDA, and CCK-8S further inhibited INMDA. Our results suggest that CCK-8S is an inhibitory modulator of NMDA receptors under normal and ethanol exposure conditions.