کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4350231 1296978 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Localization of L-type calcium channel CaV1.3 in cat lumbar spinal cord - with emphasis on motoneurons
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Localization of L-type calcium channel CaV1.3 in cat lumbar spinal cord - with emphasis on motoneurons
چکیده انگلیسی
Voltage-dependent persistent inward currents (PICs) which underlie the plateau potentials are an important intrinsic property of spinal motoneurons. Electrophysiological experiments have indicated that a subtype of the low threshold L-type calcium channel, CaV1.3, mediates this current. In mouse and turtle lumbar spinal cord it has been shown that these channel proteins are mainly found on motoneuron dendrites. In the present study we have used immunohistochemistry to locate these channels in lumbar spinal neurons, especially motoneurons, of the cat. The results indicate that CaV1.3 immunoreactivity was unevenly distributed among the laminae of the spinal grey matter. The small neurons in superficial dorsal horn (laminae I-III) were sparsely and weakly labelled, while large neurons in ventral horn were frequently and densely labelled. Groups of motoneurons in lamina IX that were immunoreactive to choline acetyltransferase also co-expressed CaV1.3. The immmunoreactivity was mainly associated with neuronal somata and proximal dendrites. Double staining with antibodies against CaV1.3 and MAP2 (a dendritic marker) showed that some fine fibres, which may include distal dendrites, were also labelled. These results in the cat spinal cord show some differences from studies in mouse and turtle motoneurons where the immunoreactivity against this channel was mainly localized to the dendrites.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 407, Issue 1, 16 October 2006, Pages 42-47
نویسندگان
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