کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4350326 | 1296981 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of C/EBPα and C/EBPβ in glial cells in vitro after inducing glial activation by different stimuli
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
We studied the involvement of two members of the CCAAT/enhancer binding proteins (C/EBPs) family of transcription factors, C/EBPα and C/EBPβ, in glial activation induced by different stimuli in rat cerebellar neuronal-glial cultures. Glial activation was induced by two types of neuronal death - excitotoxic and apoptotic. We had previously reported that these two induction mechanisms resulted in different patterns of glial activation (K. Pérez-Capote, J. Serratosa, C. Solà , Excitotoxic and apoptotic neuronal death induce different patterns of glial activation in vitro, J. Neurochem. 94 (2005) 226-237), only the former involving an inflammatory response. Glial activation was also induced by lipopolysaccharide (LPS) from E. coli, an inflammatory agent with a known direct effect on glial cells. Using immunocytochemical techniques, here we examined whether changes in C/EBPα and C/EBPβ expression are selectively associated with a determinate pattern of glial activation. Excitotoxic neuronal death increased glial C/EBPβ expression in the absence of alterations in C/EBPα expression, while no effect was observed following neuronal apoptosis. LPS treatment decreased C/EBPα and increased C/EBPβ expression in glial cells. These results implicate C/EBPs in glial activation, although these two factors appear to have different roles. C/EBPα expression decreases only in response to LPS, while C/EBPβ expression is increased by both LPS and excitotoxic neuronal death, although not by neuronal apoptosis. These results show that C/EBPβ plays an active role in glial activation, but only when this involves an inflammatory reaction, suggesting a role for C/EBPβ in neuroinflammation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 410, Issue 1, 13 December 2006, Pages 25-30
Journal: Neuroscience Letters - Volume 410, Issue 1, 13 December 2006, Pages 25-30
نویسندگان
Kamil Pérez-Capote, Josep Saura, Joan Serratosa, Carme Solà ,