Rostral parvicellular reticular formation neurons projecting to rostral ventrolateral medulla receive cardiac inputs in anesthetized rats

The rostral parvicellular reticular formation (rRFp) was explored electrophysiologically in urethane-chloralose anesthetized rats. Spontaneously-active neurons that exhibited a pulse-related activity were recorded and tested for their projections to the rostral ventrolateral medulla (RVLM). About one-third (10/29) of the rRFp neurons that exhibited a pulse-related activity were antidromically activated by RVLM stimulation with conduction velocities between 0.2-4.4 m/s and fell within the B and C fibre range. A majority (8/10) of these neurons had a low (<10 spikes/s) mean firing rate, whereas a small proportion (2/10) had a high (>15 spikes/s) mean firing rate. These findings suggest a direct pathway from the rRFp to the RVLM and suggest that neurons projecting to the RVLM receive cardiac inputs and can modulate RVLM neuronal activity.