کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4351718 | 1298078 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-level transgene expression in neurons by lentivirus with Tet-Off system
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High-level transgene expression in neurons by lentivirus with Tet-Off system High-level transgene expression in neurons by lentivirus with Tet-Off system](/preview/png/4351718.png)
چکیده انگلیسی
We developed novel lentiviral vectors by using “Tet-Off system” and succeeded in achieving high-level and neuron-specific gene transduction in vivo. One week after viral injection into the rat neostriatum, the GFP expression was almost completely neuron-specific and about 40 times higher than the expression of a conventional lentiviral vector. High transcriptional activity and neuronal specificity were sustained for up to 8 weeks. Furthermore, neuronal processes of the infected neurons were efficiently visualized by adding a plasma membrane-targeting signal to GFP. These results suggest that the present method is valuable for strong gene transduction and clear visualization of neurons in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Research - Volume 63, Issue 2, February 2009, Pages 149–154
Journal: Neuroscience Research - Volume 63, Issue 2, February 2009, Pages 149–154
نویسندگان
Hiroyuki Hioki, Eriko Kuramoto, Michiteru Konno, Hiroshi Kameda, Yasuhiro Takahashi, Takashi Nakano, Kouichi C. Nakamura, Takeshi Kaneko,