کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4356401 | 1615677 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immune cell recruitment following acoustic trauma
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کلمات کلیدی
GAPDHICAM-1RT-PCRABRNIHCD45MIP-1βF4/80 - F4 / 80Wound Healing - التیام زخمCochlea - حلزون گوشNIH, National Institutes of Health - مؤسسه ملی سلامتmacrophage inflammatory protein-1β - ماکروفاژ التهابی پروتئین 1βMacrophages - ماکروفاژها،درشت خوارهاreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسauditory brainstem response - پاسخ شنوایی مغزChemokines - کرموین ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
سیستم های حسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Acoustic trauma induces cochlear inflammation. We hypothesized that chemokines are involved in the recruitment of leukocytes as part of a wound healing response. The cochleas of NIH-Swiss mice, exposed to octave-band noise (8-16 kHz, at 118 dB) for 2 h, were examined after the termination of exposure. Leukocytes were identified immunohistochemically with antibodies to CD45 and F4/80. Gene array analysis followed by RT-PCR was performed on cochlear tissue to identify up-regulation of chemokine and adhesion molecule mRNA. The expression of the adhesion molecule ICAM-1 was also investigated immunohistochemically. Few CD45- or F4/80-positive leukocytes were observed in the non-exposed cochlea. Following acoustic trauma however, the number of CD45-positive cells was dramatically increased especially after 2 and 4 days, after which time the numbers decreased. F4/80-positive cells also increased in number over the course of a week. Gene array analysis indicated increased expression of monocyte chemoattractant protein 5 (MCP-5), monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein-1β (MIP-1β) and ICAM-1. RT-PCR, performed using primers for the individual mRNA sequences, confirmed the increased expression of MCP-1, MCP-5, MIP-1β, and ICAM-1 relative to non-exposed mice. In the normal cochlea, ICAM-1 immunohistochemical expression was observed in venules, spiral ligament fibrocytes and in endosteal cells of the scala tympani. Expression increased to include more of the spiral ligament and endosteal cells after acoustic trauma. A cochlear inflammatory response is initiated in response to acoustic trauma and involves the recruitment of circulating leukocytes to the inner ear.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hearing Research - Volume 222, Issues 1â2, December 2006, Pages 115-124
Journal: Hearing Research - Volume 222, Issues 1â2, December 2006, Pages 115-124
نویسندگان
Stephen V. Tornabene, Kunihiro Sato, Liem Pham, Peter Billings, Elizabeth M. Keithley,