Assembly of the type II secretion system

The type II secretion system is utilized by many Gram-negative bacteria to export folded proteins to the surface and/or the extracellular environment of the cell. Although the function of the system is to move proteins from the periplasm to the outside of the cell, it is a large trans-envelope structure composed of more than a dozen different proteins present in multiple copies, including peripheral, integral inner membrane and integral outer membrane proteins plus a pseudopilus stretching between them. The establishment of this structure as an integral component of the entire envelope including the peptidoglycan layer between the two membranes requires assembly. Many of the participants and processes involved in this assembly have now been established, while other aspects remain to be discovered or more fully understood.