کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4358899 1300468 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Different regulatory regions are located on the sensor domain of CpxA to fine-tune signal transduction
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Different regulatory regions are located on the sensor domain of CpxA to fine-tune signal transduction
چکیده انگلیسی

In Gram-negative bacteria, the Cpx signal transduction pathway (CpxAR) has an overall regulatory function, but the mechanism of its activation is still poorly understood. Here we investigated the impact of the periplasmic sensor domain of CpxA on signalling by mutational analysis in vitro. Substitutions (R33C and L38P) in the N-terminal region significantly increased CpxA autophosphorylation, whereas a substitution in a predicted beta sheet (E92 K), which impaired the inhibitory effect of the auxiliary CpxP protein, had no significant effect on catalytic activity. Thus, our data suggest different regions on the periplasmic domain of CpxA, which impact to modulate signal transduction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 162, Issue 4, May 2011, Pages 405–409
نویسندگان
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