کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4359114 | 1300482 | 2008 | 9 صفحه PDF | دانلود رایگان |
We defined phage genera by measuring genome relationships by the numbers of shared homologous/orthologous proteins. We used BLAST-based tools (CoreExtractor.vbs and CoreGenes) to analyze 55 fully sequenced bacteriophage genomes from the NCBI and EBI databases. This approach was first applied to the T7-related phages. Using a cut-off score of 40% homologous proteins, we identified three genera within the T7-related phages, redefined the φ29-related phages, and introduced five novel genera. The T7- and φ29-related phages were given subfamily status and named “Autographivirinae” and “Picovirinae”, respectively. Our results confirm and refine the ICTV phage classification, enable elimination of errors in public databases, and provide a straightforward tool for the molecular classification of new phage genomes.
Journal: Research in Microbiology - Volume 159, Issue 5, June 2008, Pages 406–414