کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4359168 1300487 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of novel temperature-regulated genes in the human pathogen Cryptococcus neoformans using representational difference analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Identification of novel temperature-regulated genes in the human pathogen Cryptococcus neoformans using representational difference analysis
چکیده انگلیسی
Cryptococcus neoformans is a basidiomycetous fungus and an opportunistic human pathogen that causes infections in both immunocompromised and immunocompetent hosts. The ability to survive and proliferate at the human body temperature is an essential virulence attribute of this microorganism. Representational difference analysis (RDA) was used to profile gene expression in C. neoformans grown at 37 °C or 25 °C. Contig assembly of 300 high-quality sequenced cDNAs and comparison analysis to the GenBank database led to the identification of transcripts that may be critical for both pathogen-host interactions and responses to either low or high temperature growth. Gene products involved in cell wall integrity, stress response, filamentation, oxidative metabolism, protein targeting and fatty acids metabolism were induced at 37 °C. In addition, genes related to chromatin silencing and phospholipid transport were upregulated at 25 °C. Therefore, our RDA analysis, comparing saprophytic and host temperature conditions, revealed new genes with potential involvement in C. neoformans virulence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 159, Issue 3, April 2008, Pages 221-229
نویسندگان
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