کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4359174 | 1300488 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of the alkB gene in the thermophilic Geobacillus sp. strain MH-1
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular characterization of the alkB gene in the thermophilic Geobacillus sp. strain MH-1 Molecular characterization of the alkB gene in the thermophilic Geobacillus sp. strain MH-1](/preview/png/4359174.png)
چکیده انگلیسی
An extremely thermophilic alkane-degrading bacterium, strain MH-1, was isolated from the deep subterranean petroleum reservoir in Shengli oil field, PR China. Based on its physiological characteristics and analysis of its 16S rRNA gene sequence, strain MH-1 was identified as Geobacillus stearothermophilus. Strain MH-1 was able to grow at temperatures ranging from 50 to 72 °C and effectively degraded hexadecane as the sole carbon source at 70 °C. Strain MH-1 degraded alkanes with different length chains (C12-C31) in crude oil, but it preferentially degraded middle chain-alkanes. The alkane hydroxylase system of G. stearothermophilus MH-1 was characterized. It contained at least three alkane monooxygenase gene homologs (alkB-geo1, alkB-geo4, and alkB-geo6). The AlkB-geo6 cluster, which had a high sequence similarity to the alkB2 cluster of Rhodococcus strains NRRL B-16531 and Q15, contained alkB-geo6, two rubredoxins and a putative transcriptional regulatory protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 160, Issue 8, October 2009, Pages 560-566
Journal: Research in Microbiology - Volume 160, Issue 8, October 2009, Pages 560-566
نویسندگان
Yi-Chen Liu, Tian-Tian Zhou, Jian Zhang, Lian Xu, Zhen-Hua Zhang, Qi-Rong Shen, Biao Shen,