کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4359353 1300500 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting
چکیده انگلیسی

The fingerprinting methods referred to as MSP-PCR (microsatellite-primed polymerase chain reaction) and rep-PCR (repetitive-sequence-based polymerase chain reaction) were used to discriminate between species of Botryosphaeriaceae (Fungi, Ascomycota). Several primers were tested with both methods and each primer enabled clear differentiation of all the species tested. Cluster analysis of banding patterns of the isolates corresponded well with known species delineations based on morphology and phylogenetic analysis. The methods described in this paper provide simple and rapid procedures that can be used for routine differentiation of Botryosphaeriaceae isolates at the species level. The two methods are also useful for studying intraspecific variability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 158, Issue 2, March 2007, Pages 112–121
نویسندگان
, , , ,