کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4359595 1300525 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli
چکیده انگلیسی

A collection of clinical Shiga-toxin-producing Escherichia coli (STEC) strains, mainly belonging to serotypes O26, O103, O111, O145 and O157, was characterised by a polyphasic approach including molecular serotyping, PCR-based detection of virulence factors (stx1, stx2, eae, EHEC-hlyA, saa, katP, espP), carbohydrate fermentation profiles using API50 tests and random amplification of polymorphic DNA (RAPD) fingerprinting. An RAPD protocol based on the combination of 2 primers resulted in sufficiently complex patterns enabling discrimination to the serotype level. Moreover, carbohydrate fermentation profiles obtained after evaluating up to 50 different carbohydrates led to separation of different STEC serotypes. Virulence typing results confirm the association of Shiga toxins and intimin subtypes with specific serotypes and clinical diagnosis. Clinical diagnosis of strains did not correlate with either RAPD profiles or carbohydrate fermentation patterns.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 158, Issue 7, September 2007, Pages 591–599
نویسندگان
, , , ,