کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4362894 | 1616257 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bio-molecular characterisation of indigenous Oenococcus oeni strains from Negroamaro wine
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کلمات کلیدی
AFLPNegroamaro wineRAPD-PCRMLFMLSTRandom amplified polymorphism DNAPFGELAB - آزمایشگاهOenococcus oeni - اونوکوکوس اونیLactic acid bacteria - باکتریهای اسیدلاکتیکmulti-locus sequence typing - تایپ توالی چند لوزیMalolactic fermentation - تخمیر مالولاتیکGenetic marker - نشانگر ژنتیکیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازAmplified fragment length polymorphism - پلی مورفیسم طول قطعه تقویت شده
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Bio-molecular characterisation of indigenous Oenococcus oeni strains from Negroamaro wine Bio-molecular characterisation of indigenous Oenococcus oeni strains from Negroamaro wine](/preview/png/4362894.png)
چکیده انگلیسی
The variation in the coding capacity within Oenococcus oeni can have a significant impact on wine quality. The detection of several genes involved in important metabolic pathways (i.e. citrate, sulphur and arginine metabolisms) was performed on 10 indigenous O. oeni strains from Negroamaro wine, a red table wine (Apulia, Italy). These strains were selected from 95 isolates, collected during spontaneous malolactic fermentation, according to the results of an Amplified Fragment Length Polymorphism (AFLP) analysis. A total of 16 genes were screened, most (11) of which had never previously been assayed on O. oeni. All strains possessed 10 genes encoding enzymes such as malolactic enzyme (mleA), esterase (estA), citrate lyase (citD, citE and citF), citrate transporter (maeP), α-acetolactate decarboxylase (alsD), α- acetolactate synthase (alsS), S-adenosylmethionine synthase (metK) and cystathionine β-lyase (metC) and resulted negative in the detection of genes encoding cystathionine γ-lyase (metB), ornithine transcarbamylase (arcB) and carbamate kinase (arcC). The sequence of PCR fragments of 11 genes of a representative strain (ITEM 15929) was compared to those of three reference O. oeni strains. The indigenous strain was phylogenetically more similar to PSU-1 and ATCC BAA1163 than AWRI B429. This study describes new genetic markers useful for detecting the genetic potential of O. oeni strains to contribute to aroma production and for investigating the population structure of the species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 42, September 2014, Pages 142-148
Journal: Food Microbiology - Volume 42, September 2014, Pages 142-148
نویسندگان
Maria Stella Cappello, Stefania De Domenico, Antonio Logrieco, Giacomo Zapparoli,