کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4364508 | 1616313 | 2015 | 9 صفحه PDF | دانلود رایگان |

• Production of cellulases by a new strain of Paenibacillus chitinolyticus CKS1.
• Cellobiose was the best cellulase inducer for predominant Avicelase.
• The highest Avicelase production was obtained using medicinal herbs waste.
• High temperature 80 °C and good Avicelase thermostability for industrial processes.
• Glucose and other sugars as products of cellulose hydrolysis by the strain CKS1.
A novel strain of Paenibacillus chitinolyticus CKS1 was isolated from forest soil and identified as a potent cellulase producer. The strain was able to grow on various commercial substrates including microcrystalline cellulose (Avicel), carboxymethylcellulose (CMC), and cellobiose but also on lignocellulosic waste material such as medicinal herbs waste and sawdust. On all these substrates the strain produced cellulase composed of two subunits (∼70 and ∼45 kDa) that was active on CMC, Avicel and filter paper. The maximal Avicelase activity (1.94 U/ml) was reached in a medium that contained 0.1% (w/v) of medicinal herbs waste, 3 g l−1 of yeast extract and 5.0 g l−1 of casein hydrolysate in 0.1 M phosphate buffer pH 7, after 48 h of incubation at 30 °C. The Avicelase performed optimally at 80 °C and at pH 4.8. Addition of K+ increased the Avicelase activity almost three fold and the enzyme retained 48.39% of the initial activity after 60 min. The product of Avicel and CMC hydrolysis was glucose with traces of other soluble sugars, indicating that the crude cellulase produced on waste material using the novel P. chitinolyticus strain CKS 1 could be used in eco-friendly processes of cellulose bioconversion, such as enzymatic saccharification of lignocellulosic materials in processes performed under acidophilic conditions and high temperatures.
Journal: International Biodeterioration & Biodegradation - Volume 104, October 2015, Pages 426–434