کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4365055 1616336 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Community genomics: Isolation, characterization and expression of gene coding for azoreductase
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
Community genomics: Isolation, characterization and expression of gene coding for azoreductase
چکیده انگلیسی

Soil collected from banks of the Khari-cut canal (Vatva, Ahmedabad, Gujarat, India), contaminated by industrial wastes, was used as inoculum for developing consortium enriched with dye degrading and/or tolerating bacteria. The consortium V9 was able to decolourize 100 ppm of Reactive Violet 5 within 24 h at 37 °C. The amplified azoreductase gene had a length of 537 bp containing an ORF of 178 amino acids. BLASTn and BLASTx analyses showed 97% and 98% identity to sequences of azoreductase gene of Rhodobacter sphaeroides and Bacillus cereus G9241, respectively. The azoreductase gene was cloned in expression vectors and its in vitro activity was optimized. Co-factor NADPH.Na4 (1 mM) was essential to obtain 85–90% dye degradation by cloned azoreductase gene. Escherichia coli BL21(DE3) clone PET1 was able to degrade 90% of dye within 7 min. Conversely, only 20–30% of total dye was degraded with cell extracts of control strains after 3 h.


► Development of dye decolourizing V9 consortium.
► Oxygen tolerant azoreductase from dye decolourizing enriched mixed culture.
► Expression of an azoreductase gene.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 79, April 2013, Pages 1–8
نویسندگان
, ,